Structural Similarities between Thiamin-Binding Protein and Thiaminase-I Suggest a Common Ancestor,
نویسندگان
چکیده
منابع مشابه
Structural Similarities between M Protein and Tropomyosin
M proteins of the group A streptococcus, despite their well-recognized immunological variations, have the unique capacity of maintaining a common antiphagocytic function for the organism. Antibodies to M proteins, however, are capable of neutralizing the antiphagocytic activity of these molecules (1-3). Although, immunological cross-reactions between different M types have been observed (4-6), ...
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This communication describes the development of a thiamin sensor based on the bacterial thiamin binding protein. A triple mutant (C48S, C50S, S62C) of TbpA was labeled on C62 with N-[2-(L-maleimidyl)ethyl]-7-(diethylamino)coumarin-3-carboxamide (MDCC). Thiamin binding to this protein reduced the coumarin fluorescence giving a thiamin sensor with low nanomolar sensitivity.
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ژورنال
عنوان ژورنال: Biochemistry
سال: 2008
ISSN: 0006-2960,1520-4995
DOI: 10.1021/bi7018282